WO WO2026146545A1
The present invention discloses an automated multimodal biomolecule delivery system (100) for intracellular delivery of biomolecules into biological cells cultured on nanostructured substrates, having a laser source(B) integrated with an optical assembly (C) configured to generate a circular beam spot, a positioning module(D) precisely aligning a selectable target area of a sample holder (112) directly beneath the focused circular beam spot, an imaging module(E) enables real-time monitoring of the intracellular delivery process, a display unit (103) operatively coupled with an input unit(A) allowing user-defined control of operational parameters, a processing unit (114) generating synchronised control signals to coordinate the operation of the laser and positioning module(D), enabling automated and precise biomolecule delivery, demonstrating high transfection efficiency and maintaining high cell viability across a wide range of cell types, thereby offering a versatile and efficient platform suitable for advanced biological research and therapeutic applications.
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