CN CN122445634A
本发明提供一种新型核酸农药设计、制备及递送技术。本发明提供一种具有四面体结构的RNA分子，其利用如下方法构建获得：S1：针对有害生物的靶向基因，设计9个以上siRNA或dsRNA，其中3个以上的siRNA或dsRNA为15‑35bp，6个以上的siRNA或dsRNA为9‑25bp；S2：提供与S1中siRNA互补配对的多核苷酸；S3：将15‑35bp互补配对的siRNA或dsRNA，作为四面体结构顶边的一条边，并将9‑25bp互补配对的siRNA或dsRNA，通过kissing loop序列与9‑25bp互补配对的另一siRNA或dsRNA连接，作为四面体结构底边的一条边。
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