IN IN1628MUN2014A

METHOD FOR PRODUCING MICROCHIP FOR USE IN NUCLEIC ACID AMPLIFICATION REACTION

Abstract

Provided is a method for producing a microchip for use in a nucleic acid amplification reaction which enables a simple and highly accurate analysis. Provided is a method for producing a microchip for use in a nucleic acid amplification reaction comprising: a solidification step of drying a reagent solution containing at least a portion of a substance needed for the nucleic acid amplification reaction; and an accommodation step of placing a reagent containing the solidified substance in a well which is a reaction place for the nucleic acid amplification reaction. In the microchip for use in a nucleic acid amplification reaction which is produced by the production method the substance needed for the nucleic acid amplification reaction is accommodated in a solidified state. Therefore it becomes possible to inhibit the occurrence of a non specific amplification in a nucleic acid amplification reaction and therefore it becomes possible to carry out an analysis with high accuracy.

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