IN IN349MUM2014A
[0001] The present invention relates to the production and purification of pneumococcal capsular polysaccharide conjugate. Various capsular polysaccharides from different S. pneumoniae serotypes conjugated to an immunogenic carrier protein, where the individual composition comprises serotype i.e. 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F conjugated to CRM197 individually. Therefore the object of the present invention is to provide a process for the production and purification of pneumococcal capsular polysaccharide (PnPs) from Streptococcus polysaccharide by treatment of seed inoculum and using it for large scale high yielding production of polysaccharide. Yet another object of the present invention is to provide a method for conjugation of capsular polysaccharide with carrier protein CRM197.
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