IN IN902DE2014A
[0001] The present disclosure provides novel polynucleotide sequences for high level expression in Escherichia coli and encoding recombinant SsoPox wild-type enzyme and its variant having enhanced organophosphate (OP)-hydrolyzing activity. The invention also provides a novel method to produce the recombinant enzymes in highly pure and active form in an unprecedented high yield. The invention also provide a method to generate reusable nanobiocatalyst by covalently immobilizing pure recombinant SsoPox enzymes onto magnetic nanoparticles. The reusable nanobiocatalyst can be recovered from the reaction mixture after its use and can be stored and reused multiple times to degrade the organophosphate-compounds. The recombinant SsoPox enzymes and rSsoPox-immobilized nanobiocatalyst can be used as an economical, effective and environmental-friendly OPcompound biodegrading agent.
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